3% of the population will have a titer positive at 1:320. 1:640, 1:1280 or 1:2560), this indicates more severe disease. I was immediately seen by a Rhematologist who looked at me and said you look healthy, I dont believe in blood tests. Using the previous example, Sample 12345: ANA Positive, Speckled, titer: 1:640; suggest follow-up . In 95% of patients, ANA displayed a constant speckled pattern. It can be present in MCTD (mixed connective tissue disorder), Sjogren's syndrome, lupus, and many more autoimmune disease but the pattern is MOST commonly present in …. Similarly, a speckled pattern on the ANA test can indicate almost ANY autoimmune disease, this is because a speckled pattern like a 1:80 titer level is not very significant. The ANA test gives two types of results: 1) the titre, and 2) the pattern. ANA Titer Blood Test Results Fully Explained. A speckled pattern is also found in lupus. But Titer is an overriding dx criteria for most docs. Positive ANA with 1 : 640 titer and speckled pattern and lupus anticoagulant. My Blood test results ALT 59, XXXXXXX Screen IFA Positive : XXXXXXX Pattern Homogenous XXXXXXX TITER 1 :40 Chronic fatigue+ Illness What do you think? So 1:320 is just one off of 1:160, not 160 off as you might think - not really a significant difference. The pattern just helps a doctor figure out which disease a person with positive results . 1:40, 1:80 or even 1:160), there is often no autoimmune disease. . SS-A/Ro (HEp-2000® only) Interphase: Seen with about 89% of . I saw a new rheumatologist recently who dismissed the ANA titer of 1:320 as insignificant and said since the rash. On the other hand, an ANA with a titer of 1:1,280 is a high titer, one that's often seen in lupus patients. In our laboratory, an ANA titer of 1:640 is defined as a high titer because of a 0.5% prevalence of positives in normal individuals. All of this is also regardless of pattern. Case Agent Malignancy Age Onset (time after initiation of agent) Anti-histone Ab ANA titer, pattern Anti-Ro/ Anti-La serologies Steroids used to treat: Lesion resolution (time after drug cessation) Known rheumatolo gic history: 1 1 Docetaxel and capecitabine Recurrent breast, metastatic It means you have antinuclear antibodies in your serum. Positive ANA with 1 : 640 and homogeneous pattern and positive anti-dsDNA. What does a ana titer of 1:640 speckled pattern mean and how is my life going to change. Table 2: Relationship between titration and pattern presented in positive samples, evaluated according to the gender. Sex Pattern Titer Total Code* 1:80 1:160 1:320 ≥1:640 Female Homogeneous Nuclear (HN) AC-1 1 - - 2 3 . ANA of 0, 1+ or 2+, or titers less than 1:80 (diluted 80 times) are usually unimportant. 1:1280 had it rechecked the other day and it is 1:640 speckled. Other diseases tend to have lower levels. Once a patient has a positive ANA titer, it is rarely helpful to repeat the test; ANA levels fluctuate and do not reflect . Then October 29 Low C4 6mg/dl, ANA Positive, Anti-Nuclear AB Titer 1:320, ANA pattern speckled, SS-A Antibody >8.0 AI, Lekocyte Esterase 1+. These people usually have no disease. I also have had joint pain, rash on upper torso and fatigue for 17 months. The ANA test came back at 1:640 discrete speckled pattern *which means i was chromosome positive*. 5 - 25% of healthy people with a family member suffering from lupus have a . The normal range of ANA blood test varies from laboratory to laboratory. Table 1. ANA is anti nuclear antibodies, for some reason your body is producing . The ANA titer can indicate other diseases as well, depending on how the antibodies appear under the microscope. In 2020, it was at 1:1280 titer (speckled pattern). The numbers climb quickly since one dilution doubles the finding. Certain diseases are more likely to have certain patterns . A homogenous (diffuse) pattern appears as total nuclear fluorescence and is common in people with systemic lupus. ANA pattern [10]. The ANA titer is a measure of the amount of ANA in the blood; the higher the titer, the more autoantibodies are present in the sample.. Serial Dilutions: 1:10 positive 1:20 positive 1:40 positive 1:80 positive 1:160 positive (titer reported as 1:160) 1:320 negative The Significance of ANA Pattern ANA titers and patterns can vary between laboratory testing sites due to variation in the methodology used. # A [ 5] Trace IgG, IgM, and no C1q, C3, IgA, or fibrinogen. Titer reading is determined by adding saline (salt water) to a sample of plasma (the protein-rich liquid part of blood) a certain number of times. For example, for a ratio of 1:40 or 1:80, the possibility of an autoimmune disorder is considered low. The different ANA titers (1:1280 vs. 1:320) are due to the fact that the ANA test is a very old and pretty useless test that is positive if any one or. Fine speckled pattern, chromosome-negative: Numerous small and uniform The higher the titer , the more likely the result is a "true positive" result, meaning you have significant ANAs and an autoimmune disease. What does an ANA titer of 1 1280 mean? the "titre"). I have an ana titer of 1:640 speckled pattern with Neclear. The antigenic specificity of the 2 ANA patterns observed most frequently . Check to see if the titers are increased, such as 1:320. The most frequent ANA pattern in both groups was the nuclear fine speckled pattern, which occurred at lower titer in healthy individuals than in patients with ARDs (P<0.001). 5% of the population will have a titer positive at 1:160. DX's with MS in 2000. Titer . My ENA antibody screen test was Negative. ANA is measured in how strongly it is positive, usually measured as 0 to 4+ or as a titer (the number of times a blood sample can be diluted and still be positive). Most labs will titer these samples. Dr. Neal Birnbaum answered Rheumatology 51 years experience The presence of ANA with a nucleolar pattern showed an increased relative risk (RR 1.5, 95%CI 1.03-2.3) for an associated cancer. Titers of 1:80 or lower are less likely to be significant. For example, one part of plasma mixed with 40 parts of saline is a 1:40 mixture. ANA 1:40 is found in 20 - 30% of healthy people. I just had lab work done again at Duke University, and the the ANA titer was 1:640, pattern # I was homogenous, pattern #2 was speckled. ANA reports include a titer (number) and a pattern. 10% of the population will have a titer positive at 1:80. This part of the ANA test gives an estimate of how many anti-nuclear antibodies are present (i.e. There are four different patterns that can be seen under the microscope: homogeneous, speckled, nucleolar and peripheral. ANA interpretation was the consensus of 3 independent readers using 2 HEp-2 cell slide brands at 400x mag. The combination of low titers of antibody (<1:80) and no or few signs or symptoms of disease portends a much smaller likelihood of an autoimmune disease. Differential Diagnosis. What Does a Speckled Pattern in an ANA Test Indicate? Even though your fingers don't turn blue, Raynaud's can cause red or white color changes as well. The rheumatologist ordered additional blood tests which showed a strong positive for ANA IgG . In addition, my sed. What does an ANA titer of 1 640 mean? My ANA titer in December was very weak at 1:40 with speckled pattern to now being 1:320. Even higher titers are often insignificant in patients over 60 years of age. Systemic lupus erythematosus (SLE): when active, usually a homogenous pattern on ANA or less commonly speckled, rim, or nucleolar when present in high enough titer to be . I just received a Positive ANA with a 1:640 titer in a homogenous pattern. We use cookies to give you the best possible experience on our website. This is done by taking a sample of the liquid part of the blood called the serum and diluting it with a liquid called a diluent. We use cookies to give you the best possible experience on our website. Speckled is a pattern that often occurs in lupus. However, because the significance is that the anti-SSA antibodies are present regardless of the titer endpoint, some labs do not titer the SSA/Ro pattern. Patients in the reticular group also had a higher proportion of the centromere-type nuclear ANA pattern than patients in the speckled group (20.0% vs. 2.6%, p = 0.031). However, a. Nuclear, speckled, etc. This is used to determine the possibility of an autoimmune disease being present. 1:320), the result is less clear and should be interpreted in the clinical context. Anti-extractable nuclear antigen was present in 1 healthy individual (anti-SSA/Ro) and in 52 patients with ARDs (37.7%). One multicenter study reported that 31.7% of normal individuals were ANA positive at 1:40 dilution, which was decreased to 13.3% at 1:80 and 5.0% at 1:160 dilution. The problem with the ANA is that it can be found in normal healthy people. A positive ANA test is usually reported as both a ratio (called a titer) and a pattern, such as smooth or speckled. My ANA has gone from 1:640 to 1:320. A clumpy speckled pattern may be seen with antibodies to n-RNP, Sm, and SSB/La. Lupus and other autoimmune diseases have been speculated by docs, but labs are inconsistent. Recent blood test showing VERY high positive ANA SCR, ANA TITER 1:640, PATTERN SPECKLED. I recently had an abnormal positive antinuclear antibody screen (ANA) test with a titer of 1:80. LittleFox, There are lupus patients that have speckled pattern. 2+ IgM and fibrinogen. Differential Diagnosis. A titer of 1:160 or . (9 replies) The anti-centromere, anti-scl 70, anti-sm, anti-rnp, anti-ro, anti-la, anti-ds dna, C3 and C4 compliments and RPR all came back negative/within normal limits. I just got my blood tests - Answered by a verified Doctor. The ANA (antinuclear antibody) titer blood test is used to measure the amount of ANA that happens to be in the blood. ANA 1:80 is found in 10 - 15% of healthy people. health care system with a positive ANA-DFS pattern consecutively from August 2017 to September 2018. The titer indicates how many times the lab technician had to dilute plasma from the blood to get a sample free of the antinuclear antibodies. ANA titer Action Low Any titer Observe Moderate < 1:200 > 1: 400 Observe Specific . October 15: ANA 1:640, pos Ro Antibody SS-A biopsy immunological presence and interfasce dermatititis. 1:640, 1:1280 or 1:2560), this indicates more severe disease. Anti-Scl70 and anti-RNAP-III were associated with cancer in 15 and 14%, respectively. As a result, these patients with low ANA titers need to be reevaluated less frequently—yearly unless clinical symptoms evolve to suggest an autoimmune disease. Scenario #2 • 37 years old female . He said i think your heart is just that.. an issue with your heart. ana-positive, titer >1:640, speckled/ana titer 2-1:32, ana pattern 2-homogeneous c3 180.8, rnpab, igg 1.5, crp 1.9. My ANA test came back positive and both my homogeneous and speckled are 1:640. If the ANA titre is high (e.g. ANA Titers (number) and Patterns. On Avonex ever since along with a LOT of other "symptom management" medications. I know this may be significant or not, but since it is in the right direction I am hoping for significant! . I have been having a high ANA titer speckled pattern since march 2010 that i know of. All my other rheumotologic markers are normal. In our laboratory, an ANA titer of 1:640 is defined as a "high titer" because of a 0.5% prevalence of positives in normal individuals. 1:40, 1:80 or even 1:160 . For a homogeneous ANA pattern, follow-up testing for antibodies to dsDNA is appropriate with most labs using a fluorescent slide method (CLIF), ELISA, or RIA. Our retrospective study included 425 patients from our university . ANA 1:160 is found in 5% of healthy people. An ANA of <1:40 (less than one in forty) is considered negative regardless of pattern. The presence of ANA with a homogeneous & speckled (HS) pattern was significantly associated with the absence of cancer ( p < 0.01). Methods ANA test at 1/80 screening dilution was performed in 269 sequentially selected patients with SLE diagnosis, 918 healthy individuals, and 558 patients with non-SARD conditions. Negative, 1:40 and 1:80 titers are insignificant levels. Re: AI Diagnosis Question - 1:640 ANA+ Speckled Pattern. Negative anti-dsDNA and ANCA. Patient samples are often screened for antinuclear antibodies after being diluted 1:40 and 1:160 in a buffered solution. The nuclear fine speckled pattern occurred at a titer ≤1:320 in 46 of the 54 healthy individuals with that pattern (85.2%), and the nuclear dense fine speckled pattern occurred at a titer ≥1:640 in 24 of the 39 healthy individuals with that pattern (61.5%) (P < 0.001). This pattern is NOT associated with autoimmune diseases, and from what I understand, basically rules them out. ANAs can be found in approximately 5% of the normal population, usually in low titers (low levels). Nuclear Coarse Speckled Pattern (NCSP) AC-5 1 7.2 Total 14 100 Note: *ICAP (International Consensus on ANA patterns) codes. Jul 28, 2009 Ok so my question is.. does anyone else have a high ANA discrete speckled pattern where symptoms are internal only? An ANA of 1:640 is high enough to indicate lupus or a similar autoimmune connective tissue disease, rather than the other autoimmune diseases such as thryoid which may also happen to have an elevated ANA assuming there are any of the relevant typical symptoms. We investigated the relation between DFS pattern and various diseases. (ANA titers of less than or equal to 1:40 are considered negative.) • High titer ANA >1:640 (even if asymptomatic should be evaluated as next available in Rheumatology) • Raynaud's (i.e., extremity skin color changes that include a white/numb phase) • Arthritis (persistent morning stiffness (lasting >15 minutes on more than one occasion), joint swelling Anti-nuclear Antibodies Different nucleolar patterns: (Homogenous, clumpy, fine . My question is--the pattern has always been speckled, and now for the first time it has changed to nucleolar. An ANA below 1:80 is considered normal. Often doctors don't consider an ANA positive until it hits >1:80 or >1:320. In addition, there are several types of ANAs and, when necessary, additional testing can identify the specific type of ANA (also called subtype). Female, 55, 210 lbs. Usually, the results of the ANA test are reported in titers and patterns. A titer of 1:160 is borderline and may be due to exposure to many medications, infections and environmental factors. For example, a titer of 1:640 shows a greater concentration of anti-nuclear antibodies than a titer of 1:320 or 1:160. Some laboratories add a comment to the results suggesting appropriate follow-up testing. rate is borderline high at 20 and my CRP is elevated at 1.9. Patients in the reticular group exhibited a higher proportion of nuclear ANA titers (> 1:640) than patients in the speckled group (50.0% vs. 10.8%, p < 0.001). ANA 1:640 Dr said no big deal So I had some blood done with my functional medicine Dr to try to figure out what's going on. ANA by IIF is generally reported with both a titer and a pattern. This indicates that there are antibodies present and that the test is positive. Background: Dense fine speckled (DFS) pattern in antinuclear antibody (ANA) test using indirect immunofluorescence method became to be known recently and it is detected in patients with various chronic inflammatory diseases as well as in healthy individuals. The titer is 1:40 (low) with a speckled pattern. >1:40 (greater than one in forty) is a low positive. My ESR was 9. FANA results are reported in titers and the pattern of autoantibodies made (homogeneous, speckled, centromere, etc.). Result 1: the titre. A titer of 320 means that your blood had to be diluted320 times before they got a sample that had no antinuclear antibody. Some labs only test to >1:1280. then he proceeded to take 7 vials of blood. Any sample identified as positive at the screening dilution is titered out either to endpoint or to a pre-defined dilution, depending on the laboratory's preference. If the ANA titre is low (e.g. Table 2: Prevalence of SARD diseases in ANA-DFS positive patients by titer a,b. Recently, symptoms have increased, and my ANA is 1:640 dense fine speckled. ANA 1:320 is found in 3% of healthy people. Conversely, sequentially selected individuals presenting >1/640 titer Nuclear Dense Fine Speckled (DFS . Lupus. ANA titers go up and down during . In our study, the patients with ANA titers ≥ 1:640 and initially presenting with joint pain, fever, abnormal urinalysis findings, or skin presentations were highly associated with autoimmune diseases.